Enzymatic Characteristics of In-Vitro Incorporation of P³² Orthophosphate into Human Platelet Phosphatide^[*]

Summary

The incorporation of P³² orthophosphate into phosphatide by isolated human platelets in an artificial medium was studied. The addition of homologous plasma increased the incorporation and the formation of labelled phosphatide was significantly reduced in the absence of oxygen or in the presence of cyanide, and by varying the pH from 7.4 to 5.6 and 8.6. The enzymatic character of the incorporation and the role of platelet respiration are briefly discussed.

^* Supported by Research Grant HE 05504 from the National Heart Institute of the U. S. Public Health Service.

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